Purification of a Streptococcal Bacteriocin ( Viridin B ) and Its Separation from Aipha - Hemolysin LYNN
نویسنده
چکیده
Viridin B, a bacteriocin produced by Streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000.
منابع مشابه
Interactions between Neisseria sicca and viridin B, a bacteriocin produced by Streptococcus mitis.
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